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Journal of Biomedical Optics • new

Assessment of the “cross-bridge”-induced interaction of red blood cells by optical trapping combined with microfluidics
Author(s): Kisung Lee; Christian Wagner; Alexander V. Priezzhev
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Paper Abstract

Red blood cell (RBC) aggregation is an intrinsic property of the blood that has a direct effect on the blood viscosity and circulation. Nevertheless, the mechanism behind the RBC aggregation has not been confirmed and is still under investigation with two major hypotheses, known as “depletion layer” and “cross-bridging.” We aim to ultimately understand the mechanism of the RBC aggregation and clarify both models. To measure the cell interaction in vitro in different suspensions (including plasma, isotonic solution of fibrinogen, isotonic solution of fibrinogen with albumin, and phosphate buffer saline) while moving the aggregate from one solution to another, an approach combining optical trapping and microfluidics has been applied. The study reveals evidence that RBC aggregation in plasma is at least partly due to the cross-bridging mechanism. The cell interaction strength measured in the final solution was found to be significantly changed depending on the initial solution where the aggregate was formed.

Paper Details

Date Published: 21 June 2017
PDF: 6 pages
J. Biomed. Opt. 22(9) 091516 doi: 10.1117/1.JBO.22.9.091516
Published in: Journal of Biomedical Optics Volume 22, Issue 9
Show Author Affiliations
Kisung Lee, Saarland Univ. (Germany)
M.V. Lomonosov Moscow State Univ. (Russia)
Christian Wagner, Univ. des Saarlandes (Germany)
Univ. of Luxembourg (Luxembourg)
Alexander V. Priezzhev, M.V. Lomonosov Moscow State Univ. (Russian Federation)

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