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Journal of Biomedical Optics

Assessment of decellularization of heart bioimplants using a Raman spectroscopy method
Author(s): Elena V. Timchenko; Pavel E. Timchenko; Artur Lichtenberg; Alexander Assmann; Hug Aubin; Payam Akhyari; Larisa T. Volova; Svetlana V. Pershutkina
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Paper Abstract

We report the results of experimental studies on cardiac implants using a Raman spectroscopy method (RS). Raman spectra characteristics of leaves and walls of cardiac implants were obtained; the implants were manufactured by protocols of detergent-enzymatic technique (DET) and biological, detergent-free (BIO) decellularization, using detergents (group DET) or a detergent-free, nonproteolytic, actin-disassembling regimen (BIO). There were input optical coefficients that allowed us to carry out evaluation of the protocols of DET and BIO decellularization on the basis of the concentrations of glycosaminoglycans, proteins, amides, and DNA. It was shown that during DET and BIO decellularization, composition aberrations of proteins and lipids do not occur and the integrity of the collagenous structures is preserved. It was found that during the DET decellularization, preservation of glycosaminoglycans is better than during BIO decellularization.

Paper Details

Date Published: 18 April 2017
PDF: 4 pages
J. Biomed. Opt. 22(9) 091511 doi: 10.1117/1.JBO.22.9.091511
Published in: Journal of Biomedical Optics Volume 22, Issue 9
Show Author Affiliations
Elena V. Timchenko, Samara Univ. (Russia)
Pavel E. Timchenko, Samara Univ. (Russia)
Artur Lichtenberg, Heinrich-Heine-Univ. Düsseldorf (Germany)
Alexander Assmann, Heinrich-Heine-Univ. Düsseldorf (Germany)
Hug Aubin, Heinrich-Heine-Univ. Düsseldorf (Germany)
Payam Akhyari, Heinrich-Heine-Univ. Düsseldorf (Germany)
Larisa T. Volova, Samara State Medical Univ. (Russia)
Svetlana V. Pershutkina, Samara Univ. (Russia)


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