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Journal of Biomedical Optics

Optical coherence elastography for evaluating customized riboflavin/UV-A corneal collagen crosslinking
Author(s): Manmohan Singh; Jiasong Li; Srilatha Vantipalli; Zhaolong Han; Kirill V. Larin; Michael D. Twa
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Paper Abstract

UV-induced collagen cross-linking is a promising treatment for keratoconus that stiffens corneal tissue and prevents further degeneration. Since keratoconus is generally localized, the efficacy of collagen cross-linking (CXL) treatments could be improved by stiffening only the weakened parts of the cornea. Here, we demonstrate that optical coherence elastography (OCE) can spatially resolve transverse variations in corneal stiffness. A short duration ( 1    ms ) focused air-pulse induced low amplitude ( 10    μ m ) deformations in the samples that were detected using a phase-stabilized optical coherence tomography system. A two-dimensional map of material stiffness was generated by measuring the damped natural frequency (DNF) of the air-pulse induced response at various transverse locations of a heterogeneous phantom mimicking a customized CXL treatment. After validation on the phantoms, similar OCE measurements were made on spatially selective CXL-treated in situ rabbit corneas. The results showed that this technique was able to clearly distinguish the untreated and CXL-treated regions of the cornea, where CXL increased the DNF of the cornea by 51 % . Due to the noncontact nature and minimal excitation force, this technique may be valuable for in vivo assessments of corneal biomechanical properties.

Paper Details

Date Published: 5 January 2017
PDF: 8 pages
J. Biomed. Opt. 22(9) 091504 doi: 10.1117/1.JBO.22.9.091504
Published in: Journal of Biomedical Optics Volume 22, Issue 9
Show Author Affiliations
Manmohan Singh, Univ. of Houston (United States)
Jiasong Li, Univ. of Houston (United States)
Srilatha Vantipalli, Univ. of Houston (United States)
Zhaolong Han, Univ. of Houston (United States)
Kirill V. Larin, Univ. of Houston (United States)
Baylor College of Medicine (United States)
Samara State Aerospace Univ. (Russia)
Michael D. Twa, The Univ. of Alabama at Birmingham (United States)

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