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Journal of Biomedical Optics

<italic<In vivo</italic< characterization of structural changes after topical application of glucocorticoids in healthy human skin
Author(s): Sora Jung; Jürgen Lademann; Maxim E. Darvin; Claudia Richter; Claus Bang Pedersen; Heike Richter; Sabine Schanzer; Jan Kottner; Ulrike Blume-Peytavi; Mads Almose Røpke
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Paper Abstract

Topical glucocorticoids (GC) are known to induce changes in human skin with the potential to develop skin atrophy. Here, atrophogenic effects and subsequent structural changes in the skin after topical application of GC were investigated in vivo. Sixteen healthy volunteers were topically treated daily on the forearms with clobetasol propionate, betamethasone dipropionate, and the petrolatum vehicle for 4 weeks. All treated skin areas and a nontreated control area were examined by ultrasound, optical coherence tomography, confocal laser scanning microscopy, multiphoton tomography (MPT), and resonance Raman spectroscopy at baseline 1 day after last application and 1 week after last application. Investigated parameters included stratum corneum thickness, epidermal, and full skin thickness, keratinocyte size and density, keratinocyte nucleus-to-cytoplasm ratio, skin surface classification, relative collagen and elastin signal intensity, second-harmonic generation-to-autofluorescence aging index of dermis (SAAID), and the antioxidant status of the skin. A reduction in epidermal and dermal skin thickness was observed in GC treated as well as in vehicle-treated and untreated skin areas on the volar forearm. MPT analysis showed an increased epidermal cell density and reduced cell size and nucleus-to-cytoplasm ratio and a significant increase of SAAID after GC treatment indicating a restructuring or compression of collagen fibers clinically being observed as atrophic changes.

Paper Details

Date Published: 28 July 2017
PDF: 13 pages
J. Biomed. Opt. 22(7) 076018 doi: 10.1117/1.JBO.22.7.076018
Published in: Journal of Biomedical Optics Volume 22, Issue 7
Show Author Affiliations
Sora Jung, Charité Universitätsmedizin Berlin (Germany)
Jürgen Lademann, Charité Universitätsmedizin Berlin (Germany)
Maxim E. Darvin, Charité Universitätsmedizin Berlin (Germany)
Claudia Richter, Charité Universitätsmedizin Berlin (Germany)
Claus Bang Pedersen, LEO Pharma A/S (Denmark)
Heike Richter, Charité Universitätsmedizin Berlin (Germany)
Sabine Schanzer, Charité Universitätsmedizin Berlin (Germany)
Jan Kottner, Charité Universitätsmedizin Berlin (Germany)
Ulrike Blume-Peytavi, Charité Universitätsmedizin Berlin (Germany)
Mads Almose Røpke, LEO Pharma A/S (Denmark)


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