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Journal of Biomedical Optics • new

Quantifying three-dimensional rodent retina vascular development using optical tissue clearing and light-sheet microscopy
Author(s): Jasmine N. Singh; Taylor M. Nowlin; Gregory J. Seedorf; Steven H. Abman; Douglas P. Shepherd
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Paper Abstract

Retinal vasculature develops in a highly orchestrated three-dimensional (3-D) sequence. The stages of retinal vascularization are highly susceptible to oxygen perturbations. We demonstrate that optical tissue clearing of intact rat retinas and light-sheet microscopy provides rapid 3-D characterization of vascular complexity during retinal development. Compared with flat mount preparations that dissect the retina and primarily image the outermost vascular layers, intact cleared retinas imaged using light-sheet fluorescence microscopy display changes in the 3-D retinal vasculature rapidly without the need for point scanning techniques. Using a severe model of retinal vascular disruption, we demonstrate that a simple metric based on Sholl analysis captures the vascular changes observed during retinal development in 3-D. Taken together, these results provide a methodology for rapidly quantifying the 3-D development of the entire rodent retinal vasculature.

Paper Details

Date Published: 18 July 2017
PDF: 10 pages
J. Biomed. Opt. 22(7) 076011 doi: 10.1117/1.JBO.22.7.076011
Published in: Journal of Biomedical Optics Volume 22, Issue 7
Show Author Affiliations
Jasmine N. Singh, Univ. of Colorado Denver (United States)
Univ. of Colorado Anschutz Medical Campus (United States)
Taylor M. Nowlin, Univ. of Colorado Anschutz Medical Campus (United States)
Gregory J. Seedorf, Univ. of Colorado Anschutz Medical Campus (United States)
Steven H. Abman, Univ. of Colorado Anschutz Medical Campus (United States)
Douglas P. Shepherd, Univ. of Colorado Denver (United States)
Univ. of Colorado Anschutz Medical Campus (United States)


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