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Journal of Biomedical Optics

Determining the light scattering and absorption parameters from forward-directed flux measurements in cardiac tissue
Author(s): Anthony J. Costantino; Christopher J. Hyatt; Michaela C. Kollisch-Singule; Jacques Beaumont; Bradley John Roth; Arkady M. Pertsov
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Paper Abstract

We describe a method to accurately measure the light scattering model parameters from forward-directed flux (FDF) measurements carried out with a fiber-optic probe (optrode). Improved determination of light scattering parameters will, in turn, permit better modeling and interpretation of optical mapping in the heart using voltage-sensitive dyes. Using our optrode-based system, we carried out high spatial resolution measurements of FDF in intact and homogenized cardiac tissue, as well as in intralipid-based tissue phantoms. The samples were illuminated with a broad collimated beam at 660 and 532 nm. Measurements were performed with a plunge fiber-optic probe (NA=0.22) at a spatial resolution of up to 10  μm. In the vicinity of the illuminated surface, the FDF consistently manifested a fast decaying exponent with a space constant comparable with the decay rate of ballistic photons. Using a Monte Carlo model, we obtained a simple empirical formula linking the rate of the fast exponent to the scattering coefficient, the anisotropy parameter g, and the numerical aperture of the probe. The estimates of scattering coefficient based on this formula were validated in tissue phantoms. Potential applications of optical fiber-based FDF measurements for the evaluation of optical parameters in turbid media are discussed.

Paper Details

Date Published: 14 July 2017
PDF: 11 pages
J. Biomed. Opt. 22(7) 076009 doi: 10.1117/1.JBO.22.7.076009
Published in: Journal of Biomedical Optics Volume 22, Issue 7
Show Author Affiliations
Anthony J. Costantino, Binghamton Univ. (United States)
Christopher J. Hyatt, Springfield Technical Community College (United States)
Michaela C. Kollisch-Singule, SUNY Upstate Medical Univ. (United States)
Jacques Beaumont, SUNY Upstate Medical Univ. (United States)
Bradley John Roth, Oakland Univ. (United States)
Arkady M. Pertsov, SUNY Upstate Medical Univ. (United States)

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