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Journal of Biomedical Optics • new

Multiphoton autofluorescence lifetime imaging of induced pluripotent stem cells
Author(s): Aisada Uchugonova
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Paper Abstract

The multiphoton fluorescence lifetime imaging tomograph MPTflex with its flexible 360-deg scan head, articulated arm, and tunable femtosecond laser source was employed to study induced pluripotent stem cell (iPS) cultures. Autofluorescence (AF) lifetime imaging was performed with 250-ps temporal resolution and submicron spatial resolution using time-correlated single-photon counting. The two-photon excited AF was based on the metabolic coenzymes NAD(P)H and flavin adenine dinucleotide/flavoproteins. iPS cells generated from mouse embryonic fibroblasts (MEFs) and cocultured with growth-arrested MEFs as feeder cells have been studied. Significant differences on AF lifetime signatures were identified between iPS and feeder cells as well as between their differentiating counterparts.

Paper Details

Date Published: 30 June 2017
PDF: 6 pages
J. Biomed. Opt. 22(6) 066018 doi: 10.1117/1.JBO.22.6.066018
Published in: Journal of Biomedical Optics Volume 22, Issue 6
Show Author Affiliations
Aisada Uchugonova, Univ. des Saarlandes (Germany)
JenLab GmbH (Germany)

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