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Journal of Biomedical Optics

Single-image structured illumination using Hilbert transform demodulation
Author(s): Zachary R. Hoffman; Charles A. DiMarzio
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Paper Abstract

Structured illumination microscopy (SIM) achieves sectioning at depth by removing undesired light from out-of-focus planes within a specimen. However, it generally requires at least three modulated images with discrete phase shifts of 0, 120, and 240 deg to produce sectioning. Using a Hilbert transform demodulation, it is possible to produce both sectioning and depth information relative to a reference plane (i.e., a coverslip) using only a single image. The specimen is modulated at a known frequency, and the unmodulated portion of the image is estimated. These two components are used to provide a high-quality sectioned image containing both axial and lateral information of an object. The sectioning resolution with a single image is on par with that of a control three-image SIM. We are also able to show that when used with three images of discrete phase, this method produces better contrast within a turbid media than the traditional SIM technique. Because the traditional SIM requires alignment of three different phases, small differences in optical path length can introduce strong artifacts. Using the single-image technique removes this dependency, greatly improving sectioning in turbid media. Multiple targets with various depths and opaqueness are considered, including human skin in vivo, demonstrating a quick and useful way to provide noninvasive sectioning in real time.

Paper Details

Date Published: 31 May 2017
PDF: 11 pages
J. Biomed. Opt. 22(5) 056011 doi: 10.1117/1.JBO.22.5.056011
Published in: Journal of Biomedical Optics Volume 22, Issue 5
Show Author Affiliations
Zachary R. Hoffman, Northeastern Univ. (United States)
Draper Lab. (United States)
Charles A. DiMarzio, Northeastern Univ. (United States)

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