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Journal of Biomedical Optics • Open Access • new

Line-scan focal modulation microscopy
Author(s): Shilpa Pant; Caixia Li; Zhiyuan Gong; Nanguang Chen

Paper Abstract

We report the development of a line-scan focal modulation microscope (LSFMM) that is capable of high-speed image acquisition ( > 40    fps ) with uncompromised optical sectioning capability. The improved background rejection and axial resolution of this imaging modality, enabled by focal modulation, are quantified with three-dimensional imaging data obtained from fluorescent beads. The signal-to-background ratio for the LSFMM system is one- to two-orders of magnitude higher than that for line-scanning confocal systems when imaging deep (up to 100 μm) into a turbid medium of optical properties similar to biological tissues. The imaging performance of LSFMM, in terms of both spatial and temporal resolutions, is further demonstrated with in vivo imaging experiments with live zebrafish larvae.

Paper Details

Date Published: 26 May 2017
PDF: 4 pages
J. Biomed. Opt. 22(5) 050502 doi: 10.1117/1.JBO.22.5.050502
Published in: Journal of Biomedical Optics Volume 22, Issue 5
Show Author Affiliations
Shilpa Pant, National Univ. of Singapore (Singapore)
Caixia Li, National Univ. of Singapore (Singapore)
Zhiyuan Gong, National Univ. of Singapore (Singapore)
Nanguang Chen, National Univ. of Singapore (Singapore)

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