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Journal of Biomedical Optics

Application of quantitative second-harmonic generation microscopy to posterior cruciate ligament for crimp analysis studies
Author(s): Woowon Lee; Md. Hafizur Rahman; Mariana E. Kersh; Kimani C. Toussaint
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Paper Abstract

We use second-harmonic generation (SHG) microscopy to quantitatively characterize collagen fiber crimping in the posterior cruciate ligament (PCL). The obtained SHG images are utilized to define three distinct categories of crimp organization in the PCL. Using our previously published spatial-frequency analysis, we develop a simple algorithm to quantitatively distinguish the various crimp patterns. In addition, SHG microscopy reveals both the three-dimensional structural variation in some PCL crimp patterns as well as an underlying helicity in these patterns that have mainly been observed using electron microscopy. Our work highlights how SHG microscopy could potentially be used to link the fibrous structural information in the PCL to its mechanical properties.

Paper Details

Date Published: 28 April 2017
PDF: 9 pages
J. Biomed. Opt. 22(4) 046009 doi: 10.1117/1.JBO.22.4.046009
Published in: Journal of Biomedical Optics Volume 22, Issue 4
Show Author Affiliations
Woowon Lee, Univ. of Illinois at Urbana-Champaign (United States)
Md. Hafizur Rahman, Univ. of Illinois at Urbana-Champaign (United States)
Mariana E. Kersh, Univ. of Illinois at Urbana-Champaign (United States)
Kimani C. Toussaint, Univ. of Illinois at Urbana-Champaign (United States)

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