Share Email Print
cover

Journal of Biomedical Optics

Negative dielectrophoresis spectroscopy for rare analyte quantification in biological samples
Author(s): Syed A. M. Kirmani; Fleming Dackson Gudagunti; Logeeshan Velmanickam; Dharmakeerthi Nawarathna; Ivan T. Lima
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We propose the use of negative dielectrophoresis (DEP) spectroscopy as a technique to improve the detection limit of rare analytes in biological samples. We observe a significant dependence of the negative DEP force on functionalized polystyrene beads at the edges of interdigitated electrodes with respect to the frequency of the electric field. We measured this velocity of repulsion for 0% and 0.8% conjugation of avidin with biotin functionalized polystyrene beads with our automated software through real-time image processing that monitors the Rayleigh scattering from the beads. A significant difference in the velocity of the beads was observed in the presence of as little as 80 molecules of avidin per biotin functionalized bead. This technology can be applied in the detection and quantification of rare analytes that can be useful in the diagnosis and the treatment of diseases, such as cancer and myocardial infarction, with the use of polystyrene beads functionalized with antibodies for the target biomarkers.

Paper Details

Date Published: 30 March 2017
PDF: 7 pages
J. Biomed. Opt. 22(3) 037006 doi: 10.1117/1.JBO.22.3.037006
Published in: Journal of Biomedical Optics Volume 22, Issue 3
Show Author Affiliations
Syed A. M. Kirmani, North Dakota State Univ. (United States)
COMSATS Institute of Information Technology (Pakistan)
Fleming Dackson Gudagunti, North Dakota State Univ. (United States)
Logeeshan Velmanickam, North Dakota State Univ. (United States)
Dharmakeerthi Nawarathna, North Dakota State Univ. (United States)
Ivan T. Lima, North Dakota State Univ. (United States)


© SPIE. Terms of Use
Back to Top