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Journal of Biomedical Optics • Open Access

Gray-level co-occurrence matrix analysis of several cell types in mouse brain using resolution-enhanced photothermal microscopy
Author(s): Takayoshi Kobayashi; Durga Sundaram; Kazuaki Nakata; Hiromichi Tsurui

Paper Abstract

Qualifications of intracellular structure were performed for the first time using the gray-level co-occurrence matrix (GLCM) method for images of cells obtained by resolution-enhanced photothermal imaging. The GLCM method has been used to extract five parameters of texture features for five different types of cells in mouse brain; pyramidal neurons and glial cells in the basal nucleus (BGl), dentate gyrus granule cells, cerebellar Purkinje cells, and cerebellar granule cells. The parameters are correlation, contrast, angular second moment (ASM), inverse difference moment (IDM), and entropy for the images of cells of interest in a mouse brain. The parameters vary depending on the pixel distance taken in the analysis method. Based on the obtained results, we identified that the most suitable GLCM parameter is IDM for pyramidal neurons and BGI, granule cells in the dentate gyrus, Purkinje cells and granule cells in the cerebellum. It was also found that the ASM is the most appropriate for neurons in the basal nucleus.

Paper Details

Date Published: 14 March 2017
PDF: 13 pages
J. Biomed. Opt. 22(3) 036011 doi: 10.1117/1.JBO.22.3.036011
Published in: Journal of Biomedical Optics Volume 22, Issue 3
Show Author Affiliations
Takayoshi Kobayashi, The Univ. of Electro-Communications (Japan)
Japan Science and Technology Agency (Japan)
National Chiao-Tung Univ. (Taiwan)
Durga Sundaram, The Univ. of Electro-Communications (Japan)
SASTRA Univ. (India)
Kazuaki Nakata, The Univ. of Electro-Communications (Japan)
Hiromichi Tsurui, Juntendo Univ. (Japan)

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