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Journal of Biomedical Optics

Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy
Author(s): Jing-Yu Huang; Lun-Zhang Guo; Jing-Zun Wang; Tse-Chung Li; Hsin-Jung Lee; Po-Kai Chiu; Lung-Han Peng; Tzu-Ming Liu
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Paper Abstract

Harmonic generation microscopy (HGM) has become one unique tool of optical virtual biopsy for the diagnosis of cancer and the in vivo cytometry of leukocytes. Without labeling, HGM can reveal the submicron features of tissues and cells in vivo. For deep imaging depth and minimal invasiveness, people commonly adopt 1100- to 1300-nm femtosecond laser sources. However, those lasers are typically based on bulky oscillators whose performances are sensitive to environmental conditions. We demonstrate a fiber-based 1150-nm femtosecond laser source, with 6.5-nJ pulse energy, 86-fs pulse width, and 11.25-MHz pulse repetition rate. It was obtained by a bismuth borate or magnesium-doped periodically poled lithium niobate (MgO:PPLN) mediated frequency doubling of the 2300-nm solitons, generated from an excitation of 1550-nm femtosecond pulses on a large mode area photonic crystal fiber. Combined with a home-built laser scanned microscope and a tailor-made frame grabber, we achieve a pulse-per-pixel HGM imaging in vivo at a 30-Hz frame rate. This integrated solution has the potential to be developed as a stable HGM system for routine clinical use.

Paper Details

Date Published: 8 March 2017
PDF: 8 pages
J. Biomed. Opt. 22(3) 036008 doi: 10.1117/1.JBO.22.3.036008
Published in: Journal of Biomedical Optics Volume 22, Issue 3
Show Author Affiliations
Jing-Yu Huang, National Taiwan Univ. (Taiwan)
Lun-Zhang Guo, National Taiwan Univ. (Taiwan)
Jing-Zun Wang, National Taiwan Univ. (Taiwan)
Tse-Chung Li, National Taiwan Univ. (Taiwan)
Hsin-Jung Lee, National Taiwan Univ. (Taiwan)
Po-Kai Chiu, National Applied Research Labs. (Taiwan)
Lung-Han Peng, National Taiwan Univ. (Taiwan)
Tzu-Ming Liu, National Taiwan Univ. (Taiwan)
Univ. of Macau (China)

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