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Journal of Biomedical Optics

Motion characterization scheme to minimize motion artifacts in intravital microscopy
Author(s): Sungon Lee; Gabriel Courties; Matthias Nahrendorf; Ralph Weissleder; Claudio Vinegoni
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Paper Abstract

Respiratory- and cardiac-induced motion artifacts pose a major challenge for in vivo optical imaging, limiting the temporal and spatial imaging resolution in fluorescence laser scanning microscopy. Here, we present an imaging platform developed for in vivo characterization of physiologically induced axial motion. The motion characterization system can be straightforwardly implemented on any conventional laser scanning microscope and can be used to evaluate the effectiveness of different motion stabilization schemes. This method is particularly useful to improve the design of novel tissue stabilizers and to facilitate stabilizer positioning in real time, therefore facilitating optimal tissue immobilization and minimizing motion induced artifacts.

Paper Details

Date Published: 2 March 2017
PDF: 7 pages
J. Biomed. Opt. 22(3) 036005 doi: 10.1117/1.JBO.22.3.036005
Published in: Journal of Biomedical Optics Volume 22, Issue 3
Show Author Affiliations
Sungon Lee, Hanyang Univ. (Korea, Republic of)
Gabriel Courties, Massachusetts General Hospital (United States)
Matthias Nahrendorf, Massachusetts General Hospital (United States)
Ralph Weissleder, Massachusetts General Hospital (United States)
Claudio Vinegoni, Massachusetts General Hospital (United States)


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