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Journal of Biomedical Optics

Segmentation of nucleus and cytoplasm of a single cell in three-dimensional tomogram using optical coherence tomography
Author(s): Chia-Kai Chang; Chien-Chung Tsai; Wan-Yi Hsu; Jau-Shiuh Chen; Yi-Hua Liao; Yi-Shuan Sheen; Jin-Bon Hong; Ming-Yi Lin; Jeng-Wei Tjiu; Sheng-Lung Huang
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Paper Abstract

A random rayburst sampling (RRBS) framework was developed to detect the nucleus and cell membrane boundaries in three-dimensional (3-D) space. Raw images were acquired through a full-field optical coherence tomography system with submicron resolution—i.e., 0.8    μ m in lateral and 0.9    μ m in axial directions. The near-isometric resolution enables 3-D segmentation of a nucleus and cell membrane for determining the volumetric nuclear-to-cytoplasmic (N/C) ratio of a single cell. The RRBS framework was insensitive to the selection of seeds and image pixel noise. The robustness of the RRBS framework was verified through the convergence of the N/C ratio searching algorithm. The relative standard deviation of the N/C ratio between different randomly selected seed sets was only 2%. This technique is useful for various in vitro assays on single-cell analyses.

Paper Details

Date Published: 2 March 2017
PDF: 12 pages
J. Biomed. Opt. 22(3) 036003 doi: 10.1117/1.JBO.22.3.036003
Published in: Journal of Biomedical Optics Volume 22, Issue 3
Show Author Affiliations
Chia-Kai Chang, National Taiwan Univ. (Taiwan)
Chien-Chung Tsai, National Taiwan Univ. (Taiwan)
Wan-Yi Hsu, National Taiwan Univ. (Taiwan)
Jau-Shiuh Chen, National Taiwan Univ. Hospital (Taiwan)
Yi-Hua Liao, National Taiwan Univ. Hospital (Taiwan)
Yi-Shuan Sheen, National Taiwan Univ. Hospital (Taiwan)
Jin-Bon Hong, National Taiwan Univ. Hospital (Taiwan)
Ming-Yi Lin, National Taiwan Univ. Hospital (Taiwan)
Jeng-Wei Tjiu, National Taiwan Univ. Hospital (Taiwan)
Sheng-Lung Huang, National Taiwan Univ. (Taiwan)

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