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Journal of Biomedical Optics • Open Access

Infrared microspectroscopic determination of collagen cross-links in articular cartilage
Author(s): Lassi Rieppo; Harri T. Kokkonen; Katariina A. M. Kulmala; Vuokko Kovanen; Mikko J. Lammi; Juha Toyras; Simo Saarakkala

Paper Abstract

Collagen forms an organized network in articular cartilage to give tensile stiffness to the tissue. Due to its long half-life, collagen is susceptible to cross-links caused by advanced glycation end-products. The current standard method for determination of cross-link concentrations in tissues is the destructive high-performance liquid chromatography (HPLC). The aim of this study was to analyze the cross-link concentrations nondestructively from standard unstained histological articular cartilage sections by using Fourier transform infrared (FTIR) microspectroscopy. Half of the bovine articular cartilage samples ( n = 27 ) were treated with threose to increase the collagen cross-linking while the other half ( n = 27 ) served as a control group. Partial least squares (PLS) regression with variable selection algorithms was used to predict the cross-link concentrations from the measured average FTIR spectra of the samples, and HPLC was used as the reference method for cross-link concentrations. The correlation coefficients between the PLS regression models and the biochemical reference values were r = 0.84 ( p < 0.001 ), r = 0.87 ( p < 0.001 ) and r = 0.92 ( p < 0.001 ) for hydroxylysyl pyridinoline (HP), lysyl pyridinoline (LP), and pentosidine (Pent) cross-links, respectively. The study demonstrated that FTIR microspectroscopy is a feasible method for investigating cross-link concentrations in articular cartilage.

Paper Details

Date Published: 14 March 2017
PDF: 9 pages
J. Biomed. Opt. 22(3) 035007 doi: 10.1117/1.JBO.22.3.035007
Published in: Journal of Biomedical Optics Volume 22, Issue 3
Show Author Affiliations
Lassi Rieppo, Univ. of Oulu (Finland)
Univ. of Eastern Finland (Finland)
Harri T. Kokkonen, South Karelia Central Hospital (Finland)
Katariina A. M. Kulmala, Univ. of Eastern Finland (Finland)
Vuokko Kovanen, Univ. of Jyväskylä (Finland)
Mikko J. Lammi, Umeå Univ. (Sweden)
Xi’an Jiaotong Univ. Health Science Ctr. (China)
Juha Toyras, Univ. of Eastern Finland (Finland)
Kuopio Univ. Hospital (Finland)
Simo Saarakkala, Univ. of Oulu (Finland)
Oulu Univ. Hospital (Finland)

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