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Journal of Biomedical Optics • Open Access

Analysis of optically anisotropic properties of biological tissues under stretching based on differential Mueller matrix formalism
Author(s): Hao-Wei Chen; Chih-Ling Huang; Yu-Lung Lo; You-Ren Chang

Paper Abstract

The optical properties of biological tissues under stretching are investigated using a full-field ellipsometry technique based on a differential Mueller matrix formalism. Traditional photoelastic-based formalism for extracting the linear birefringence (LB) properties of stretched anisotropic optical samples ignores the effects of the other optical properties of the sample. By contrast, in the formalism proposed in this study, the LB, linear dichroism (LD), circular birefringence (CB), circular dichroism (CD), and depolarization (Dep) properties are fully decoupled. Simulations are performed to evaluate the performance of the two formalisms in extracting the LB properties of optically anisotropic samples with different degrees of Dep, CB, LD, and CD. The practical feasibility of the proposed all-parameter decoupled formalism is then demonstrated using chicken breast muscle tissue. In general, the results show that both formalisms provide a reliable LB measurement performance for healthy chicken breast tissue under stretching. However, while the LB-only formalism has good robustness toward scattering, its measurement performance is seriously degraded for samples with high CB. Thus, of the two formalisms, the proposed all-parameter decoupled formalism provides a more effective approach for examining the anisotropic properties of biological tissues under stretching.

Paper Details

Date Published: 11 March 2017
PDF: 9 pages
J. Biomed. Opt. 22(3) 035006 doi: 10.1117/1.JBO.22.3.035006
Published in: Journal of Biomedical Optics Volume 22, Issue 3
Show Author Affiliations
Hao-Wei Chen, National Cheng Kung Univ. (Taiwan)
Chih-Ling Huang, Kaohsiung Medical Univ. (Taiwan)
Yu-Lung Lo, National Cheng Kung Univ. (Taiwan)
You-Ren Chang, National Cheng Kung Univ. (Taiwan)

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