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Journal of Biomedical Optics • Open Access

Methylene-blue aided rapid confocal laser endomicroscopy of breast cancer
Author(s): Khushi Vyas; Michael Hughes; Daniel R. Leff; Guang-Zhong Yang

Paper Abstract

Breast conserving surgery allows complete tumor resection while maintaining acceptable cosmesis for patients. Safe and rapid intraoperative margin assessment during the procedure is important to establish the completeness of tumor excision and minimizes the need for reoperation. Confocal laser endomicroscopy has demonstrated promise for real-time intraoperative margin assessment using acriflavine staining, but it is not approved for routine in-human use. We describe a custom high-speed line-scan confocal laser endomicroscopy (LS-CLE) system at 660 nm that enables high-resolution histomorphological imaging of breast tissue stained with methylene-blue, an alternative fluorescent stain for localizing sentinel nodes during breast surgery. Preliminary imaging results on freshly excised human breast tissue specimens are presented, demonstrating the potential of methylene-blue aided rapid LS-CLE to determine the oncological status of surgical margins in-vivo.

Paper Details

Date Published: 9 February 2017
PDF: 4 pages
J. Biomed. Opt. 22(2) 020501 doi: 10.1117/1.JBO.22.2.020501
Published in: Journal of Biomedical Optics Volume 22, Issue 2
Show Author Affiliations
Khushi Vyas, Imperial College London (United Kingdom)
Michael Hughes, Imperial College London (United Kingdom)
Daniel R. Leff, Imperial College London (United Kingdom)
Imperial College Healthcare NHS Trust (United Kingdom)
Guang-Zhong Yang, Imperial College London (United Kingdom)

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