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Journal of Biomedical Optics • new

Real-time detection of antibiotic activity by measuring nanometer-scale bacterial deformation
Author(s): Rafael Iriya; Karan Syal; Wenwen Jing; Manni Mo; Hui Yu; Shelley E. Haydel; Shaopeng Wang; Nongjian Tao
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Paper Abstract

Diagnosing antibiotic-resistant bacteria currently requires sensitive detection of phenotypic changes associated with antibiotic action on bacteria. Here, we present an optical imaging-based approach to quantify bacterial membrane deformation as a phenotypic feature in real-time with a nanometer scale ( 9    nm ) detection limit. Using this approach, we found two types of antibiotic-induced membrane deformations in different bacterial strains: polymyxin B induced relatively uniform spatial deformation of Escherichia coli O157:H7 cells leading to change in cellular volume and ampicillin-induced localized spatial deformation leading to the formation of bulges or protrusions on uropathogenic E. coli CFT073 cells. We anticipate that the approach will contribute to understanding of antibiotic phenotypic effects on bacteria with a potential for applications in rapid antibiotic susceptibility testing.

Paper Details

Date Published: 12 December 2017
PDF: 9 pages
J. Biomed. Opt. 22(12) 126002 doi: 10.1117/1.JBO.22.12.126002
Published in: Journal of Biomedical Optics Volume 22, Issue 12
Show Author Affiliations
Rafael Iriya, Arizona State Univ. (United States)
Karan Syal, Arizona State Univ. (United States)
Wenwen Jing, Arizona State Univ. (United States)
Manni Mo, Arizona State Univ. (United States)
Hui Yu, Arizona State Univ. (United States)
Nanjing Univ. (China)
Shelley E. Haydel, Arizona State Univ. (United States)
Shaopeng Wang, The Biodesign Institute (United States)
Nanjing Univ. (China)
Nongjian Tao, Arizona State Univ. (United States)
Nanjing Univ. (China)


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