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Journal of Biomedical Optics • new

Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue
Author(s): Isa Carneiro; Sónia Carvalho; Rui Henrique; Luís Oliveira; Valery V. Tuchin
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Paper Abstract

The optical dispersion and water content of human liver were experimentally studied to estimate the optical dispersions of tissue scatterers and dry matter. Using temporal measurements of collimated transmittance [ T c ( t ) ] of liver samples under treatment at different glycerol concentrations, free water and diffusion coefficient ( D gl ) of glycerol in liver were found as 60.0% and 8.2 × 10 7    cm 2 / s , respectively. Bound water was calculated as the difference between the reported total water of 74.5% and found free water. The optical dispersion of liver was calculated from the measurements of refractive index (RI) of tissue samples made for different wavelengths between 400 and 1000 nm. Using liver and water optical dispersions at 20°C and the free and total water, the dispersions for liver scatterers and dry matter were calculated. The estimated dispersions present a decreasing behavior with wavelength. The dry matter dispersion shows higher RI values than liver scatterers, as expected. Considering 600 nm, dry matter has an RI of 1.508, whereas scatterers have an RI of 1.444. These dispersions are useful to characterize the RI matching mechanism in optical clearing treatments, provided that [ T c ( t ) ] and thickness measurements are performed during treatment. The knowledge of D gl is also important for living tissue cryoprotection applications.

Paper Details

Date Published: 5 December 2017
PDF: 10 pages
J. Biomed. Opt. 22(12) 125002 doi: 10.1117/1.JBO.22.12.125002
Published in: Journal of Biomedical Optics Volume 22, Issue 12
Show Author Affiliations
Isa Carneiro, IPO-PORTO (Portugal)
Sónia Carvalho, IPO-PORTO (Portugal)
Rui Henrique, IPO-PORTO (Portugal)
Institute of Biomedical Sciences Abel Salazar, Univ. of Porto (Portugal)
Luís Oliveira, Instituto Politécnico do Porto (Portugal)
Instituto Superior de Engenharia do Porto (Portugal)
Valery V. Tuchin, Saratov State Univ. (Russian Federation)
Institute of Precise Mechanics and Control RAS (Russian Federation)
ITMO Univ. (Russian Federation)

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