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Journal of Biomedical Optics

Activatable clinical fluorophore-quencher antibody pairs as dual molecular probes for the enhanced specificity of image-guided surgery
Author(s): Girgis Obaid; Bryan Q. Spring; Shazia Bano; Tayyaba Hasan
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Paper Abstract

The emergence of fluorescently labeled therapeutic antibodies has given rise to molecular probes for image-guided surgery. However, the extraneous interstitial presence of an unbound and nonspecifically accumulated probe gives rise to false-positive detection of tumor tissue and margins. Thus, the concept of tumor-cell activation of smart probes provides a potentially superior mechanism of delineating tumor margins as well as small tumor deposits. The combination of molecular targeting with intracellular activation circumvents the presence of extracellular, nonspecific signals of targeted probe accumulation. Here, we present a demonstration of the clinical antibodies cetuximab (cet, anti-EGFR mAb) and trastuzumab (trast, anti-HER-2 mAb) conjugated to Alexa Fluor molecules and IRDye QC-1 quencher optimized at the ratio of

Paper Details

Date Published: 29 August 2017
PDF: 6 pages
J. Biomed. Opt. 22(12) 121607 doi: 10.1117/1.JBO.22.12.121607
Published in: Journal of Biomedical Optics Volume 22, Issue 12
Show Author Affiliations
Girgis Obaid, Wellman Ctr. for Photomedicine (United States)
Bryan Q. Spring, Northeastern Univ. (United States)
Shazia Bano, Wellman Ctr. for Photomedicine (United States)
Tayyaba Hasan, Wellman Ctr. for Photomedicine (United States)
Harvard Univ. (United States)
Massachusetts Institute of Technology (United States)

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