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Journal of Biomedical Optics • Open Access • new

Wide-field spectrally resolved quantitative fluorescence imaging system: toward neurosurgical guidance in glioma resection
Author(s): Yijing Xie; Maria Thom; Michael Ebner; Victoria Wykes; Adrien Desjardins; Anna Miserocchi; Sebastien Ourselin; Andrew W. McEvoy; Tom Vercauteren

Paper Abstract

In high-grade glioma surgery, tumor resection is often guided by intraoperative fluorescence imaging. 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) provides fluorescent contrast between normal brain tissue and glioma tissue, thus achieving improved tumor delineation and prolonged patient survival compared with conventional white-light-guided resection. However, commercially available fluorescence imaging systems rely solely on visual assessment of fluorescence patterns by the surgeon, which makes the resection more subjective than necessary. We developed a wide-field spectrally resolved fluorescence imaging system utilizing a Generation II scientific CMOS camera and an improved computational model for the precise reconstruction of the PpIX concentration map. In our model, the tissue’s optical properties and illumination geometry, which distort the fluorescent emission spectra, are considered. We demonstrate that the CMOS-based system can detect low PpIX concentration at short camera exposure times, while providing high-pixel resolution wide-field images. We show that total variation regularization improves the contrast-to-noise ratio of the reconstructed quantitative concentration map by approximately twofold. Quantitative comparison between the estimated PpIX concentration and tumor histopathology was also investigated to further evaluate the system.

Paper Details

Date Published: 14 November 2017
PDF: 14 pages
J. Biomed. Opt. 22(11) 116006 doi: 10.1117/1.JBO.22.11.116006
Published in: Journal of Biomedical Optics Volume 22, Issue 11
Show Author Affiliations
Yijing Xie, Univ. College London (United Kingdom)
Maria Thom, Univ. College London (United Kingdom)
Michael Ebner, Univ. College London (United Kingdom)
Victoria Wykes, Univ. College London (United Kingdom)
Adrien Desjardins, Univ. College London (United Kingdom)
Anna Miserocchi, Univ. College London Hospital (United Kingdom)
Sebastien Ourselin, Univ. College London (United Kingdom)
Andrew W. McEvoy, Univ. College London (United Kingdom)
Tom Vercauteren, Univ. College London (United Kingdom)

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