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Journal of Biomedical Optics • new

Localized surface plasmon resonance–based fiber-optic sensor for the detection of triacylglycerides using silver nanoparticles
Author(s): Anjli Baliyan; Sruthi Prasood Usha; Banshi D. Gupta; Rani Gupta; Enakshi Khular Sharma
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Paper Abstract

A label-free technique for the detection of triacylglycerides by a localized surface plasmon resonance (LSPR)–based biosensor is demonstrated. An LSPR-based fiber-optic sensor probe is fabricated by immobilizing lipase enzyme on silver nanoparticles (Ag-NPs) coated on an unclad segment of a plastic clad optical fiber. The size and shape of nanoparticles were characterized by high-resolution transmission electron microscopy and UV–visible spectroscopy. The peak absorbance wavelength changes with concentration of triacylglycerides surrounding the sensor probe, and sensitivity is estimated from shift in the peak absorbance wavelength as a function of concentration. The fabricated sensor was characterized for the concentration of triacylglyceride solution in the range 0 to 7 mM. The sensor shows the best sensitivity at a temperature of 37°C and pH 7.4 of the triacylglycerides emulsion with a response time of 40 s. A sensitivity of 28.5    nm / mM of triacylglyceride solution is obtained with a limit of detection of 0.016 mM in the entire range of triacylglycerides. This compact biosensor shows good selectivity, stability, and reproducibility in the entire physiological range of triacylglycerides and is well-suited to real-time online monitoring and remote sensing.

Paper Details

Date Published: 27 October 2017
PDF: 10 pages
J. Biomed. Opt. 22(10) 107001 doi: 10.1117/1.JBO.22.10.107001
Published in: Journal of Biomedical Optics Volume 22, Issue 10
Show Author Affiliations
Anjli Baliyan, Univ. of Delhi South Campus (India)
Sruthi Prasood Usha, Indian Institute of Technology Delhi (India)
Banshi D. Gupta, Indian Institute of Technology Delhi (India)
Rani Gupta, Univ. of Delhi South Campus (India)
Enakshi Khular Sharma, Univ. of Delhi South Campus (India)

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