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Journal of Biomedical Optics

Analysis of red blood cell parameters by Talbot-projected fringes
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Paper Abstract

Red blood cell (RBC) anomalies are significant symptoms for identification of health disorders and several blood diseases, which involve the modification of the parameters and biophysical characteristics of such cells. The aim of this study is to measure the three-dimensional phase information of healthy RBCs and their parameters, such as cell diameter, thickness, and hemoglobin (Hb) content, using Talbot-projected fringes. The Talbot image of linear grating is projected onto an RBC slide. The deformed grating lines due to the shape and refractive index of RBCs are recorded by a CCD camera through a 20 × microscope objective. Hilbert transform is used to extract the phase image from the deformed projected grating lines. Experimentally calculated values of diameter ( 8.2    μ m ), thickness ( 2.7    μ m ), and Hb content ( 28.7    pg / cell ) are well within the limits available in the literature. The proposed system is robust and user-friendly and performs the imaging of RBCs with high axial and lateral resolution ( 2.19    μ m ).

Paper Details

Date Published: 13 October 2017
PDF: 8 pages
J. Biomed. Opt. 22(10) 106009 doi: 10.1117/1.JBO.22.10.106009
Published in: Journal of Biomedical Optics Volume 22, Issue 10
Show Author Affiliations
Shilpi Agarwal, Instrument Design and Development Ctr. (India)
Varun Kumar, Instrument Design and Development Ctr. (India)
Chandra Shakher, Instrument Design and Development Ctr. (India)

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