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Journal of Biomedical Optics

Spatiotemporal image correlation analysis of blood flow in branched vessel networks of zebrafish embryos
Author(s): Nicolo G. Ceffa; Ilaria Cesana; Maddalena Collini; Laura D’Alfonso; Silvia Carra; Franco Cotelli; Laura Sironi; Giuseppe Chirico
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Paper Abstract

Ramification of blood circulation is relevant in a number of physiological and pathological conditions. The oxygen exchange occurs largely in the capillary bed, and the cancer progression is closely linked to the angiogenesis around the tumor mass. Optical microscopy has made impressive improvements in in vivo imaging and dynamic studies based on correlation analysis of time stacks of images. Here, we develop and test advanced methods that allow mapping the flow fields in branched vessel networks at the resolution of 10 to 20    μ m . The methods, based on the application of spatiotemporal image correlation spectroscopy and its extension to cross-correlation analysis, are applied here to the case of early stage embryos of zebrafish.

Paper Details

Date Published: 13 October 2017
PDF: 7 pages
J. Biomed. Opt. 22(10) 106008 doi: 10.1117/1.JBO.22.10.106008
Published in: Journal of Biomedical Optics Volume 22, Issue 10
Show Author Affiliations
Nicolo G. Ceffa, Univ. degli Studi di Milano-Bicocca (Italy)
Ilaria Cesana, Univ. degli Studi di Milano-Bicocca (Italy)
Maddalena Collini, Univ. degli Studi di Milano-Bicocca (Italy)
Istituto di Scienze applicata e Sistemi Intelligenti (Italy)
Laura D’Alfonso, Univ. degli Studi di Milano-Bicocca (Italy)
Silvia Carra, Univ. degli Studi di Milano (Italy)
Franco Cotelli, Univ. degli Studi di Milano (Italy)
Laura Sironi, Univ. degli Studi di Milano-Bicocca (Italy)
Giuseppe Chirico, Univ. degli Studi di Milano-Bicocca (Italy)
Istituto di Scienze applicata e Sistemi Intelligenti (Italy)


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