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Journal of Biomedical Optics

Raman spectral signatures of cervical exfoliated cells from liquid-based cytology samples
Author(s): Padraig Kearney; Damien Traynor; Franck Bonnier; Fiona M. Lyng; John J. O'Leary; Cara Martin
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Paper Abstract

It is widely accepted that cervical screening has significantly reduced the incidence of cervical cancer worldwide. The primary screening test for cervical cancer is the Papanicolaou (Pap) test, which has extremely variable specificity and sensitivity. There is an unmet clinical need for methods to aid clinicians in the early detection of cervical precancer. Raman spectroscopy is a label-free objective method that can provide a biochemical fingerprint of a given sample. Compared with studies on infrared spectroscopy, relatively few Raman spectroscopy studies have been carried out to date on cervical cytology. The aim of this study was to define the Raman spectral signatures of cervical exfoliated cells present in liquid-based cytology Pap test specimens and to compare the signature of high-grade dysplastic cells to each of the normal cell types. Raman spectra were recorded from single exfoliated cells and subjected to multivariate statistical analysis. The study demonstrated that Raman spectroscopy can identify biochemical signatures associated with the most common cell types seen in liquid-based cytology samples; superficial, intermediate, and parabasal cells. In addition, biochemical changes associated with high-grade dysplasia could be identified suggesting that Raman spectroscopy could be used to aid current cervical screening tests.

Paper Details

Date Published: 30 October 2017
PDF: 10 pages
J. Biomed. Opt. 22(10) 105008 doi: 10.1117/1.JBO.22.10.105008
Published in: Journal of Biomedical Optics Volume 22, Issue 10
Show Author Affiliations
Padraig Kearney, Coombe Women & Infants Univ. Hospital (Ireland)
Trinity College Dublin (Ireland)
Damien Traynor, Focas Research Institute (Ireland)
Dublin Institute of Technology (Ireland)
Franck Bonnier, Focas Research Institute (Ireland)
Dublin Institute of Technology (Ireland)
Univ. François Rabelais (France)
Fiona M. Lyng, Focas Research Institute (Ireland)
Dublin Institute of Technology (Ireland)
John J. O'Leary, Coombe Women & Infants Univ. Hospital (Ireland)
Trinity College Dublin (Ireland)
Cara Martin, Coombe Women & Infants Univ. Hospital (Ireland)
Trinity College Dublin (Ireland)


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