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Journal of Biomedical Optics • Open Access • new

Single fiber reflectance spectroscopy calibration

Paper Abstract

To accurately determine sample optical properties using single fiber reflectance spectroscopy (SFR), an absolute calibration of the reflectance is required. We investigated two SFR calibration methods, using a calibrated mirror and using the Fresnel reflection at the fiber tip as a reference. We compared these to commonly used calibration methods, using either Intralipid-20% in combination with Monte Carlo simulations or Spectralon as a reference. The Fresnel reflection method demonstrated the best reproducibility and yielded the most reliable result. We therefore recommend the Fresnel reflection method for the measured absolute reflectance calibration of SFR.

Paper Details

Date Published: 30 October 2017
PDF: 4 pages
J. Biomed. Opt. 22(10) 100502 doi: 10.1117/1.JBO.22.10.100502
Published in: Journal of Biomedical Optics Volume 22, Issue 10
Show Author Affiliations
Xu U. Zhang, Academisch Medisch Centrum (The Netherlands)
Anouk L. Post, Academisch Medisch Centrum (The Netherlands)
Dirk J. Faber, Academisch Medisch Centrum (The Netherlands)
Ton G. van Leeuwen, Academisch Medisch Centrum (The Netherlands)
Henricus J. C. M. Sterenborg, Academisch Medisch Centrum (The Netherlands)
Netherlands Cancer Institute (The Netherlands)

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