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Journal of Biomedical Optics • Open Access

Evaluating anesthetic protocols for functional blood flow imaging in the rat eye
Author(s): Eric M. Moult; WooJhon Choi; David A. Boas; Bernhard Baumann; Allen C. Clermont; Edward P. Feener; James G. Fujimoto

Paper Abstract

The purpose of this study is to evaluate the suitability of five different anesthetic protocols (isoflurane, isoflurane–xylazine, pentobarbital, ketamine–xylazine, and ketamine–xylazine–vecuronium) for functional blood flow imaging in the rat eye. Total retinal blood flow was measured at a series of time points using an ultrahigh-speed Doppler OCT system. Additionally, each anesthetic protocol was qualitatively evaluated according to the following criteria: (1) time-stability of blood flow, (2) overall rate of blood flow, (3) ocular immobilization, and (4) simplicity. We observed that different anesthetic protocols produced markedly different blood flows. Different anesthetic protocols also varied with respect to the four evaluated criteria. These findings suggest that the choice of anesthetic protocol should be carefully considered when designing and interpreting functional blood flow studies in the rat eye.

Paper Details

Date Published: 5 January 2017
PDF: 6 pages
J. Biomed. Opt. 22(1) 016005 doi: 10.1117/1.JBO.22.1.016005
Published in: Journal of Biomedical Optics Volume 22, Issue 1
Show Author Affiliations
Eric M. Moult, Research Lab. of Electronics (United States)
Massachusetts Institute of Technology (United States)
WooJhon Choi, Research Lab. of Electronics (United States)
Massachusetts Institute of Technology (United States)
David A. Boas, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Harvard Medical School (United States)
Bernhard Baumann, Massachusetts Institute of Technology (United States)
Medizinische Univ. Wien (Austria)
Research Lab. of Electronics (United States)
Allen C. Clermont, Joslin Diabetes Ctr. (United States)
Edward P. Feener, Joslin Diabetes Ctr. (United States)
James G. Fujimoto, Massachusetts Institute of Technology (United States)
Research Lab. of Electronics (United States)


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