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Journal of Biomedical Optics

Poly(3-hydroxybutyrate) anabolism in Cupriavidus necator cultivated at various carbon-to-nitrogen ratios: insights from single-cell Raman spectroscopy
Author(s): Zhanhua Tao; Pengfei Zhang; Zhaojun Qin; Yong-Qing Li; Guiwen Wang
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Paper Abstract

Cupriavidus necator accumulates large amounts of poly(3-hydroxybutyrate) (PHB), a biodegradable substitute for petroleum-based plastics, under certain nutrient conditions. Conventional solvent-extraction-based methods for PHB quantification only obtain average information from cell populations and, thus, mask the heterogeneity among individual cells. Laser tweezers Raman spectroscopy (LTRS) was used to monitor dynamic changes in the contents of PHB, nucleic acids, and proteins in

Paper Details

Date Published: 16 September 2016
PDF: 7 pages
J. Biomed. Opt. 21(9) 097005 doi: 10.1117/1.JBO.21.9.097005
Published in: Journal of Biomedical Optics Volume 21, Issue 9
Show Author Affiliations
Zhanhua Tao, Guangxi Academy of Sciences (China)
Pengfei Zhang, Washington Univ. in St. Louis (United States)
Zhaojun Qin, Guangxi Academy of Sciences (China)
Yong-Qing Li, East Carolina Univ. (United States)
Guiwen Wang, Guangxi Academy of Sciences (China)


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