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Journal of Biomedical Optics

Evaluation of Raman spectroscopy in comparison to commonly performed dengue diagnostic tests
Author(s): Saranjam Khan; Muhammad Khurram; Hina Ali; Rahat Ullah; Ajmal Khan; Arshad Mahmood; Mushtaq Ahmed
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Paper Abstract

This study demonstrates the evaluation of Raman spectroscopy as a rapid diagnostic test in comparison to commonly performed tests for an accurate detection of dengue fever in human blood sera. Blood samples of 104 suspected dengue patients collected from Holy Family Hospital, Rawalpindi, Pakistan, have been used in this study. Out of 104 samples, 52 (50%) were positive based on immunoglobulin G (IgG), whereas 54 (52%) were positive based on immunoglobulin M (IgM) antibody tests. For the determination of the diagnostic capabilities of Raman spectroscopy, accuracy, sensitivity, specificity and false positive rate have been calculated in comparison to normally performed IgM and IgG captured enzyme-linked immunosorbent assay tests. Accuracy, precision, specificity, and sensitivity for Raman spectroscopy in comparison to IgM were found to be 66%, 70%, 72%, and 61%, whereas based on IgG they were 47%, 46%, 52%, and 43%, respectively.

Paper Details

Date Published: 26 September 2016
PDF: 4 pages
J. Biomed. Opt. 21(9) 095005 doi: 10.1117/1.JBO.21.9.095005
Published in: Journal of Biomedical Optics Volume 21, Issue 9
Show Author Affiliations
Saranjam Khan, National Institute for Lasers and Optronics (Pakistan)
Muhammad Khurram, Rawalpindi Medical College (Pakistan)
Hina Ali, National Institute for Lasers and Optronics (Pakistan)
Rahat Ullah, National Institute for Lasers and Optronics (Pakistan)
Ajmal Khan, COMSATS Institute of Information Technology (Pakistan)
Arshad Mahmood, National Institute for Lasers and Optronics (Pakistan)
Mushtaq Ahmed, National Institute for Lasers and Optronics (Pakistan)


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