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Journal of Biomedical Optics

Profiling of individual human red blood cells under osmotic stress using defocusing microscopy
Author(s): Paula Magda S. Roma; Livia Siman; Barbara Hissa; Ubirajara Agero; Erika M. Braga; Oscar N. Mesquita
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Paper Abstract

We use a quantitative phase imaging technique, defocusing microscopy (DM), to measure morphological, chemical, and mechanical parameters of individual red blood cells (RBCs) immersed in solutions with different osmolalities. We monitor the RBCs’ radius, volume, surface area, sphericity index, and hemoglobin content and concentration. The complete shape of cells is recovered and the effects of their adhesion to the glass substrate are observed. Finally, membrane fluctuation measurements give us information about the cells deformability.

Paper Details

Date Published: 21 September 2016
PDF: 4 pages
J. Biomed. Opt. 21(9) 090505 doi: 10.1117/1.JBO.21.9.090505
Published in: Journal of Biomedical Optics Volume 21, Issue 9
Show Author Affiliations
Paula Magda S. Roma, Univ. Federal de Minas Gerais (Brazil)
Livia Siman, Univ. Federal de Minas Gerais (Brazil)
Barbara Hissa, Univ. of Chicago (United States)
Ubirajara Agero, Univ. Federal de Minas Gerais (Brazil)
Erika M. Braga, Univ. Federal de Minas Gerais (Brazil)
Oscar N. Mesquita, Univ. Federal de Minas Gerais (Brazil)

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