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Journal of Biomedical Optics

Low-level laser therapy induces an upregulation of collagen gene expression during the initial process of bone healing: a microarray analysis
Author(s): Carla Roberta Tim; Paulo Sérgio Bossini; Hueliton Wilian Kido; Iran Malavazi; Marcia Regina von Zeska Kress; Marcelo Falsarella Carazzolle; Ana Cláudia Rennó; Nivaldo Antonio Parizotto
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Paper Abstract

This study investigates the histological modifications produced by low level laser therapy (LLLT) on the first day of bone repair, as well as evaluates the LLLT effects on collagen expression on the site of a fracture. Twenty Wistar rats were distributed into a control group (CG) and a laser group (LG). Laser irradiation of Ga-Al-As laser 830 nm, 30 mW, 94 s, 2.8 J was performed in five sessions. Animals were euthanized on day 5 postsurgery. Histopathological analysis showed that LLLT was able to increase deposition of granulation tissue and newly formed bone at the site of the injury. In addition, picrosirius analysis showed that collagen fiber organization in the LG was enhanced compared to CG. Microarray analysis demonstrated that LLLT produced an upregulation type I collagen (COL-I). Immunohistochemical analysis revealed that the subjects that were treated presented a higher immunoexpression of COL-I. Our findings indicated that LLLT improves bone healing by producing a significant increase in the expression of collagen genes.

Paper Details

Date Published: 19 August 2016
PDF: 9 pages
J. Biomed. Opt. 21(8) 088001 doi: 10.1117/1.JBO.21.8.088001
Published in: Journal of Biomedical Optics Volume 21, Issue 8
Show Author Affiliations
Carla Roberta Tim, Univ. Federal de São Paulo (Brazil)
Paulo Sérgio Bossini, Univ. Federal de São Paulo (Brazil)
Hueliton Wilian Kido, Univ. Federal de São Paulo (Brazil)
Iran Malavazi, Univ. Federal de São Carlos (Brazil)
Marcia Regina von Zeska Kress, Univ. de São Paulo (Brazil)
Marcelo Falsarella Carazzolle, Univ. Estadual de Campinas (Brazil)
Brazilian National Ctr. for Research in Energy and Materials (Brazil)
Ana Cláudia Rennó, Univ. Federal de São Paulo (Brazil)
Nivaldo Antonio Parizotto, UFSCAR (Brazil)


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