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Journal of Biomedical Optics • Open Access

Miniature standoff Raman probe for neurosurgical applications
Author(s): Oliver A. C. Stevens; Joanne Hutchings; William Gray; Rosa Louise Vincent; John C. Day

Paper Abstract

Removal of intrinsic brain tumors is a delicate process, where a high degree of specificity is required to remove all of the tumor tissue without damaging healthy brain. The accuracy of this process can be greatly enhanced by intraoperative guidance. Optical biopsies using Raman spectroscopy are a minimally invasive and lower-cost alternative to current guidance methods. A miniature Raman probe for performing optical biopsies of human brain tissue is presented. The probe allows sampling inside a conventional stereotactic brain biopsy system: a needle of length 200 mm and inner diameter of 1.8 mm. By employing a miniature stand-off Raman design, the probe removes the need for any additional components to be inserted into the brain. Additionally, the probe achieves a very low internal silica background while maintaining good collection of Raman signal. To illustrate this, the probe is compared with a Raman probe that uses a pair of optical fibers for collection. The miniature stand-off Raman probe is shown to collect a comparable number of Raman scattered photons, but the Raman signal to background ratio is improved by a factor of five at Raman shifts below ∼500  cm−1. The probe’s suitability for use on tissue is demonstrated by discriminating between different types of healthy porcine brain tissue.

Paper Details

Date Published: 10 August 2016
PDF: 6 pages
J. Biomed. Opt. 21(8) 087002 doi: 10.1117/1.JBO.21.8.087002
Published in: Journal of Biomedical Optics Volume 21, Issue 8
Show Author Affiliations
Oliver A. C. Stevens, Univ. of Bristol (United Kingdom)
Joanne Hutchings, Univ. of Bristol (United Kingdom)
Gloucestershire Hospitals NHS Foundation Trust (United Kingdom)
William Gray, Cardiff Univ. (United Kingdom)
Rosa Louise Vincent, Pfizer Inc. (United States)
John C. Day, Univ. of Bristol (United Kingdom)


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