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Journal of Biomedical Optics • Open Access

Quantitative index imaging of coculture cells by scanning focused refractive index microscopy
Author(s): Teng-Qian Sun; Qing Ye; Fen Hu; Shi-ke Liu; Xiao-Wan Wang; Jin Wang; Zhi-Chao Deng; Jian-Chun Mei; Wen-Yuan Zhou; Chun-Ping Zhang; Xin-Yu Wang; Lei-Ting Pan; Jian-Guo Tian

Paper Abstract

We report the quantitative refractive index (RI) imaging of cocultured cells in their living environment by scanning focused refractive index microscopy (SFRIM). Mouse microglial cells and synovial cells are cocultured on the top surface of a trapezoid prism. The RI imaging of living cells is obtained in a reflection-type method. The RI information is deduced with the simple derivative total internal reflection method, where a complex retrieval algorithm or reconstruction process is unnecessary. The outline of each cell is determined according to the RI value compared with that of the immersion liquid. The cocultured cells can be discriminated in the RI image. The measurement is nondestructive and label-free. The experimental results prove that SFRIM is a promising tool in the field of biological optics.

Paper Details

Date Published: 26 August 2016
PDF: 6 pages
J. Biomed. Opt. 21(8) 086016 doi: 10.1117/1.JBO.21.8.086016
Published in: Journal of Biomedical Optics Volume 21, Issue 8
Show Author Affiliations
Teng-Qian Sun, Nankai Univ. (China)
Qing Ye, Nankai Univ. (China)
Fen Hu, Nankai Univ. (China)
Shi-ke Liu, Nankai Univ. (China)
Xiao-Wan Wang, Nankai Univ. (China)
Jin Wang, Nankai Univ. (China)
Zhi-Chao Deng, Nankai Univ. (China)
Jian-Chun Mei, Nankai Univ. (China)
Wen-Yuan Zhou, Nankai Univ. (China)
Chun-Ping Zhang, Nankai Univ. (China)
Xin-Yu Wang, Nankai Univ. (China)
Lei-Ting Pan, Nankai Univ. (China)
Jian-Guo Tian, Nankai Univ. (China)

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