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Journal of Biomedical Optics

Near-infrared indocyanine dye permits real-time characterization of both venous and lymphatic circulation
Author(s): Toshikazu Kurahashi; Katsuyuki Iwatsuki; Tetsuro Onishi; Tetsuya Arai; Katsunori Teranishi; Hitoshi Hirata
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Paper Abstract

We investigated the optical properties of a near-infrared (NIR) fluorochrome, di-β-cyclodextrin-binding indocyanine derivative (TK-1), and its pharmacokinetic differences with indocyanine green (ICG). TK-1 was designed to have hydrophilic cyclodextrin molecules and, thus, for higher water solubility and smaller particle sizes than the plasma protein-bound ICG. We compared optical properties such as the absorption and fluorescence spectra, quantum yield, and photostability between both dyes in vitro. In addition, we subcutaneously injected a 1 mM solution of TK-1 or ICG into the hind footpad of rats and observed real-time NIR fluorescence intensities in their femoral veins and accompanying lymphatics at the exposed groin site to analyze the dye pharmacokinetics. These optical experiments demonstrated that TK-1 has high water solubility, a low self-aggregation tendency, and high optical and chemical stabilities. Our in vivo imaging showed that TK-1 was transported via peripheral venous flow and lymphatic flow, whereas ICG was drained only through lymphatics. The results of this study showed that lymphatic and venous transport can be differentially regulated and is most likely influenced primarily by particle size, and that TK-1 can enable real-time NIR fluorescence imaging of whole fluids and solute movement via both microvessels and lymphatics, which conventional ICG cannot achieve.

Paper Details

Date Published: 17 August 2016
PDF: 11 pages
J. Biomed. Opt. 21(8) 086009 doi: 10.1117/1.JBO.21.8.086009
Published in: Journal of Biomedical Optics Volume 21, Issue 8
Show Author Affiliations
Toshikazu Kurahashi, Nagoya Univ. (Japan)
Anjo Kosei Hospital (Japan)
Katsuyuki Iwatsuki, Nagoya Univ. (Japan)
Tetsuro Onishi, Nagoya Univ. (Japan)
Tetsuya Arai, Gifu Prefectural Tajimi Hospital (Japan)
Katsunori Teranishi, Mie Univ. (Japan)
Hitoshi Hirata, Nagoya Univ. (Japan)

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