Share Email Print
cover

Journal of Biomedical Optics

Fluorescence Talbot microscope using incoherent source
Author(s): Yangyang Sun; Shuo Pang
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Fluorescence Talbot microscope is a scalable field-of-view (FOV) imaging platform, which takes advantage of the phase sensitivity of the self-image of a periodic structure. Such a system can maintain the microscopic resolution and extend the FOV for the whole slide (15  mm×15  mm) scanning. Previously reported Talbot fluorescence systems, tabletop and on-chip device alike, rely on the coherence of the illumination source, limiting their potential applications in low-resource setting environment. A more cost-effective setup using a light-emitting diode, which has an area of 4  mm2 and a full width at half maximum of 16 nm in wavelength, is demonstrated. Compared to the illumination that is spatially filtered by a single pinhole, our system has achieved an illumination intensity that is 357 times higher. The reconstructed image quality is comparable to that of a 10× microscope objective. Various samples, such as fluorescent beads, green fluorescence protein-labeled HeLa cells, and a mouse kidney slide, were reconstructed by the system.

Paper Details

Date Published: 8 August 2016
PDF: 5 pages
J. Biomed. Opt. 21(8) 086003 doi: 10.1117/1.JBO.21.8.086003
Published in: Journal of Biomedical Optics Volume 21, Issue 8
Show Author Affiliations
Yangyang Sun, CREOL, The College of Optics and Photonics, Univ. of Central Florida (United States)
Univ. of Central Florida (United States)
Shuo Pang, CREOL, The College of Optics and Photonics, Univ. of Central Florida (United States)
Univ. of Central Florida (United States)


© SPIE. Terms of Use
Back to Top