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Journal of Biomedical Optics

Investigation of injection dose and camera integration time on quantifying pharmacokinetics of a Cy5.5-GX1 probe with dynamic fluorescence imaging in vivo
Author(s): Yunpeng Dai; Xueli Chen; Jipeng Yin; Xiaoyu Kang; Guodong Wang; Xianghan Zhang; Yongzhan Nie; Kaichun Wu; Jimin Liang
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Paper Abstract

The aim of this article is to investigate the influence of a tracer injection dose (ID) and camera integration time (IT) on quantifying pharmacokinetics of Cy5.5-GX1 in gastric cancer BGC-823 cell xenografted mice. Based on three factors, including whether or not to inject free GX1, the ID of Cy5.5-GX1, and the camera IT, 32 mice were randomly divided into eight groups and received 60-min dynamic fluorescence imaging. Gurfinkel exponential model (GEXPM) and Lammertsma simplified reference tissue model (SRTM) combined with a singular value decomposition analysis were used to quantitatively analyze the acquired dynamic fluorescent images. The binding potential (Bp) and the sum of the pharmacokinetic rate constants (SKRC) of Cy5.5-GX1 were determined by the SRTM and EXPM, respectively. In the tumor region, the SKRC value exhibited an obvious trend with change in the tracer ID, but the Bp value was not sensitive to it. Both the Bp and SKRC values were independent of the camera IT. In addition, the ratio of the tumor-to-muscle region was correlated with the camera IT but was independent of the tracer ID. Dynamic fluorescence imaging in conjunction with a kinetic analysis may provide more quantitative information than static fluorescence imaging, especially for

Paper Details

Date Published: 3 August 2016
PDF: 6 pages
J. Biomed. Opt. 21(8) 086001 doi: 10.1117/1.JBO.21.8.086001
Published in: Journal of Biomedical Optics Volume 21, Issue 8
Show Author Affiliations
Yunpeng Dai, Xidian Univ. (China)
Xueli Chen, Xidian Univ. (China)
Jipeng Yin, Fourth Military Medical Univ. (China)
Xiaoyu Kang, Fourth Military Medical Univ. (China)
Guodong Wang, Fourth Military Medical Univ. (China)
Xianghan Zhang, Xidian Univ. (China)
Yongzhan Nie, Fourth Military Medical Univ. (China)
Kaichun Wu, Fourth Military Medical Univ. (China)
Jimin Liang, Xidian Univ. (China)

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