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Journal of Biomedical Optics

Optical clearing for luminal organ imaging with ultrahigh-resolution optical coherence tomography
Author(s): Yanmei Liang; Wu Yuan; Jessica Mavadia-Shukla; Xingde Li
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Paper Abstract

The imaging depth of optical coherence tomography (OCT) in highly scattering biological tissues (such as luminal organs) is limited, particularly for OCT operating at shorter wavelength regions (such as around 800 nm). For the first time, the optical clearing effect of the mixture of liquid paraffin and glycerol on luminal organs was explored with ultrahigh-resolution spectral domain OCT at 800 nm. Ex vivo studies were performed on pig esophagus and bronchus, and guinea pig esophagus with different volume ratios of the mixture. We found that the mixture of 40% liquid paraffin had the best optical clearing effect on esophageal tissues with a short effective time of ∼10  min, which means the clearing effect occurs about 10 min after the application of the clearing agent. In contrast, no obvious optical clearing effect was identified on bronchus tissues.

Paper Details

Date Published: 23 June 2016
PDF: 6 pages
J. Biomed. Opt. 21(8) 081211 doi: 10.1117/1.JBO.21.8.081211
Published in: Journal of Biomedical Optics Volume 21, Issue 8
Show Author Affiliations
Yanmei Liang, Johns Hopkins Univ. (United States)
Nankai Univ. (China)
Wu Yuan, Johns Hopkins Univ. (United States)
Jessica Mavadia-Shukla, Johns Hopkins Univ. (United States)
Xingde Li, Johns Hopkins Univ. (United States)

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