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Journal of Biomedical Optics • Open Access

Optical clearing of skin enhanced with hyaluronic acid for increased contrast of optoacoustic imaging
Author(s): Anton V. Liopo; Richard Su; Dmitri A. Tsyboulski; Alexander A. Oraevsky

Paper Abstract

Enhanced delivery of optical clearing agents (OCA) through skin may improve sensitivity of optical and optoacoustic (OA) methods of imaging, sensing, and monitoring. This report describes a two-step method for enhancement of light penetration through skin. Here, we demonstrate that topical application of hyaluronic acid (HA) improves skin penetration of hydrophilic and lipophilic OCA and thus enhances their performance. We examined the OC effect of 100% polyethylene and polypropylene glycols (PPGs) and their mixture after pretreatment by HA, and demonstrated significant increase in efficiency of light penetration through skin. Increased light transmission resulted in a significant increase of OA image contrast in vitro. Topical pretreatment of skin for about 30 min with 0.5% HA in aqueous solution offers effective delivery of low molecular weight OCA such as a mixture of PPG-425 and polyethylene glycol (PEG)-400. The developed approach of pretreatment by HA prior to application of clearing agents (PEG and PPG) resulted in a ∼47-fold increase in transmission of red and near-infrared light and significantly enhanced contrast of OA images.

Paper Details

Date Published: 27 May 2016
PDF: 9 pages
J. Biomed. Opt. 21(8) 081208 doi: 10.1117/1.JBO.21.8.081208
Published in: Journal of Biomedical Optics Volume 21, Issue 8
Show Author Affiliations
Anton V. Liopo, TomoWave Labs, Inc. (United States)
Richard Su, TomoWave Labs, Inc. (United States)
Univ. of Houston (United States)
Dmitri A. Tsyboulski, TomoWave Labs, Inc. (United States)
Alexander A. Oraevsky, TomoWave Labs, Inc. (United States)
Univ. of Houston (United States)

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