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Journal of Biomedical Optics

Decalcification using ethylenediaminetetraacetic acid for clear microstructure imaging of cochlea through optical coherence tomography
Author(s): Jaeyul Lee; Kanghae Kim; Ruchire Eranga Wijesinghe; Doekmin Jeon; Sang Heun Lee; Mansik Jeon; Jeong Hun Jang
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Paper Abstract

The aim of this study was to analyze the effectiveness of decalcification using ethylenediaminetetraacetic acid (EDTA) as an optical clearing method to enhance the depth visibility of internal soft tissues of cochlea. <italic<Ex vivo</italic< mouse and guinea pig cochlea samples were soaked in EDTA solutions for decalcification, and swept source optical coherence tomography (OCT) was used as imaging modality to monitor the decalcified samples consecutively. The monitored noninvasive cross-sectional images showed that the mouse and guinea pig cochlea samples had to be decalcified for subsequent 7 and 14 days, respectively, to obtain the optimal optical clearing results. Using this method, difficulties in imaging of internal cochlea microstructures of mice could be evaded. The obtained results verified that the depth visibility of the decalcified <italic<ex vivo</italic< samples was enhanced.

Paper Details

Date Published: 16 March 2016
PDF: 7 pages
J. Biomed. Opt. 21(8) 081204 doi: 10.1117/1.JBO.21.8.081204
Published in: Journal of Biomedical Optics Volume 21, Issue 8
Show Author Affiliations
Jaeyul Lee, Kyungpook National Univ. (Korea, Republic of)
Kanghae Kim, Kyungpook National Univ. (Korea, Republic of)
Ruchire Eranga Wijesinghe, Kyungpook National Univ. (Republic of Korea)
Doekmin Jeon, Kyungpook National Univ. (Korea, Republic of)
Sang Heun Lee, Daegu Veterans Hospital (Korea, Republic of)
Mansik Jeon, Kyungpook National Univ. (Korea, Republic of)
Jeong Hun Jang, Ajou Univ. School of Medicine (Korea, Republic of)

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