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Journal of Biomedical Optics • Open Access

Rapid and prodium iodide-compatible optical clearing method for brain tissue based on sugar/sugar-alcohol
Author(s): Tingting Yu; Yisong Qi; Jianru Wang; Wei Feng; Jianyi Xu; Jingtan Zhu; Yingtao Yao; Hui Gong; Qingming Luo; Dan Zhu

Paper Abstract

The developed optical clearing methods show great potential for imaging of large-volume tissues, but these methods present some nonnegligible limitations such as complexity of implementation and long incubation times. In this study, we tried to screen out rapid optical clearing agents by means of molecular dynamical simulation and experimental demonstration. According to the optical clearing potential of sugar and sugar-alcohol, we further evaluated the improvement in the optical clearing efficacy of mouse brain samples, imaging depth, fluorescence preservation, and linear deformation. The results showed that drops of sorbitol, sucrose, and fructose could quickly make the mouse brain sample transparent within 1 to 2 min, and induce about threefold enhancement in imaging depth. The former two could evidently enhance the fluorescence intensity of green fluorescent protein (GFP) and prodium iodide (PI) nuclear dye. Fructose could significantly increase the fluorescence intensity of PI, but slightly decrease the fluorescence intensity of GFP. Even though the three agents caused some shrinkage in samples, the contraction in horizontal and longitudinal directions are almost the same.

Paper Details

Date Published: 11 March 2016
PDF: 7 pages
J. Biomed. Opt. 21(8) 081203 doi: 10.1117/1.JBO.21.8.081203
Published in: Journal of Biomedical Optics Volume 21, Issue 8
Show Author Affiliations
Tingting Yu, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Yisong Qi, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Jianru Wang, Huazhong Univ. of Science and Technology (China)
Britton Chance Ctr. for Biomedical Photonics (China)
Wei Feng, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Jianyi Xu, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Jingtan Zhu, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Yingtao Yao, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Hui Gong, Huazhong Univ. of Science and Technology (China)
Britton Chance Ctr. for Biomedical Photonics (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)
Britton Chance Ctr. for Biomedical Photonics (China)
Dan Zhu, Huazhong Univ. of Science and Technology (China)
Britton Chance Ctr. for Biomedical Photonics (China)


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