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Journal of Biomedical Optics • Open Access

Combined effects of scattering and absorption on laser speckle contrast imaging

Paper Abstract

Several variables may affect the local contrast values in laser speckle contrast imaging (LSCI), irrespective of relative motion. It has been suggested that the optical properties of the moving fluid and surrounding tissues can affect LSCI values. However, a detailed study of this has yet to be presented. In this work, we examined the combined effects of the reduced scattering and absorption coefficients on LSCI. This study employs fluid phantoms with different optical properties that were developed to mimic whole blood with varying hematocrit levels. These flow phantoms were imaged with an LSCI system developed for this study. The only variable parameter was the optical properties of the flowing fluid. A negative linear relationship was seen between the changes in contrast and changes in reduced scattering coefficient, absorption coefficient, and total attenuation coefficient. The change in contrast observed due to an increase in the scattering coefficient was greater than what was observed with an increase in the absorption coefficient. The results indicate that optical properties affect contrast values and that they should be considered in the interpretation of LSCI data.

Paper Details

Date Published: 1 July 2016
PDF: 7 pages
J. Biomed. Opt. 21(7) 076002 doi: 10.1117/1.JBO.21.7.076002
Published in: Journal of Biomedical Optics Volume 21, Issue 7
Show Author Affiliations
Kosar Khaksari, Michigan Technological Univ. (United States)
Sean J. Kirkpatrick, Michigan Technological Univ. (United States)

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