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Journal of Biomedical Optics • Open Access

Fluorescence lifetime measurements in heterogeneous scattering medium
Author(s): Goro Nishimura; Kamlesh Awasthi; Daisuke Furukawa

Paper Abstract

Fluorescence lifetime in heterogeneous multiple light scattering systems is analyzed by an algorithm without solving the diffusion or radiative transfer equations. The algorithm assumes that the optical properties of medium are constant in the excitation and emission wavelength regions. If the assumption is correct and the fluorophore is a single species, the fluorescence lifetime can be determined by a set of measurements of temporal point-spread function of the excitation light and fluorescence at two different concentrations of the fluorophore. This method is not dependent on the heterogeneity of the optical properties of the medium as well as the geometry of the excitation–detection on an arbitrary shape of the sample. The algorithm was validated by an indocyanine green fluorescence in phantom measurements and demonstrated by an

Paper Details

Date Published: 25 July 2016
PDF: 10 pages
J. Biomed. Opt. 21(7) 075013 doi: 10.1117/1.JBO.21.7.075013
Published in: Journal of Biomedical Optics Volume 21, Issue 7
Show Author Affiliations
Goro Nishimura, Hokkaido Univ. (Japan)
Kamlesh Awasthi, Hokkaido University (Japan)
Daisuke Furukawa, Hokkaido University (Japan)

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