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Journal of Biomedical Optics • Open Access

Polarized light imaging specifies the anisotropy of light scattering in the superficial layer of a tissue
Author(s): Steven L. Jacques; Stéphane Roussel; Ravikant Venkata Samatham

Paper Abstract

This report describes how optical images acquired using linearly polarized light can specify the anisotropy of scattering (g) and the ratio of reduced scattering [μ's=μs(1−g)] to absorption (μa), i.e., N′=μ's/μa. A camera acquired copolarized (HH) and crosspolarized (HV) reflectance images of a tissue (skin), which yielded images based on the intensity ( I=HH+HV) and difference (Q=HHHV) of reflectance images. Monte Carlo simulations generated an analysis grid (or lookup table), which mapped Q and I into a grid of g versus N′, i.e., g(Q,I) and N′(Q,I). The anisotropy g is interesting because it is sensitive to the submicrometer structure of biological tissues. Hence, polarized light imaging can monitor shifts in the submicrometer (50 to 1000 nm) structure of tissues. The Q values for forearm skin on two subjects (one Caucasian, one pigmented) were in the range of 0.046±0.007 (24), which is the mean±SD for 24 measurements on 8 skin sites×3 visible wavelengths, 470, 524, and 625 nm, which indicated g values of 0.67±0.07 (24).

Paper Details

Date Published: 10 May 2016
PDF: 6 pages
J. Biomed. Opt. 21(7) 071115 doi: 10.1117/1.JBO.21.7.071115
Published in: Journal of Biomedical Optics Volume 21, Issue 7
Show Author Affiliations
Steven L. Jacques, Oregon Health & Science Univ. (United States)
Stéphane Roussel, Univ. Paris-Sud 11 (France)
Ravikant Venkata Samatham, Oregon Health & Science Univ. (United States)

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