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Journal of Biomedical Optics • Open Access

Polarization response of second-harmonic images for different collagen spatial distributions
Author(s): Francisco J. Ávila; Oscar del Barco; Juan M. Bueno

Paper Abstract

The response to polarization of second-harmonic generation (SHG) microscopy images of samples with different collagen distributions (quasialigned, partially organized, and nonorganized) has been analyzed. A linear decay relationship between the external arrangement and polarization sensitivity was found. SHG signal from nonorganized samples presented a large structural dispersion and a weak dependence with incident polarization. Polarization dependence is also associated with the internal organization of the collagen fibers, directly related to the ratio of hyperpolarizabilities ρ. This parameter can experimentally be computed from the modulation of the SHG signal. The results show that both external and internal collagen structures are closely related. This provides a tool to obtain information of internal properties from the polarimetric response of the external spatial distribution of collagen, which might be useful in clinical diagnosis of pathologies related to changes in collagen structure.

Paper Details

Date Published: 22 June 2016
PDF: 7 pages
J. Biomed. Opt. 21(6) 066015 doi: 10.1117/1.JBO.21.6.066015
Published in: Journal of Biomedical Optics Volume 21, Issue 6
Show Author Affiliations
Francisco J. Ávila, Lab. de Óptica Univ. de Murcia (Spain)
Oscar del Barco, Lab. de Óptica Univ. de Murcia (Spain)
Juan M. Bueno, Lab. de Óptica Univ. de Murcia (Spain)

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