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Journal of Biomedical Optics

Grueneisen relaxation photoacoustic microscopy in vivo
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Paper Abstract

Grueneisen relaxation photoacoustic microscopy (GR-PAM) can achieve optically defined axial resolution, but it has been limited to ex vivo demonstrations so far. Here, we present the first in vivo image of a mouse brain acquired with GR-PAM. To induce the GR effect, an intensity-modulated continuous-wave laser was employed to heat absorbing objects. In phantom experiments, an axial resolution of 12.5  μm was achieved, which is sixfold better than the value achieved by conventional optical-resolution PAM. This axial-resolution improvement was further demonstrated by imaging a mouse brain in vivo, where significantly narrower axial profiles of blood vessels were observed. The in vivo demonstration of GR-PAM shows the potential of this modality for label-free and high-resolution anatomical and functional imaging of biological tissues.

Paper Details

Date Published: 8 June 2016
PDF: 6 pages
J. Biomed. Opt. 21(6) 066005 doi: 10.1117/1.JBO.21.6.066005
Published in: Journal of Biomedical Optics Volume 21, Issue 6
Show Author Affiliations
Jun Ma, Washington Univ. in St. Louis (United States)
Junhui Shi, Washington Univ. in St. Louis (United States)
Pengfei Hai, Washington Univ. in St. Louis (United States)
Yong Zhou, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


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