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Journal of Biomedical Optics

Finite-difference time-domain-based optical microscopy simulation of dispersive media facilitates the development of optical imaging techniques
Author(s): Di Zhang; Ilker Capoglu; Yue Li; Lusik Cherkezyan; John Chandler; Graham Spicer; Hariharan Subramanian; Allen Taflove; Vadim Backman
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Paper Abstract

Combining finite-difference time-domain (FDTD) methods and modeling of optical microscopy modalities, we previously developed an open-source software package called Angora, which is essentially a “microscope in a computer.” However, the samples being simulated were limited to nondispersive media. Since media dispersions are common in biological samples (such as cells with staining and metallic biomarkers), we have further developed a module in Angora to simulate samples having complicated dispersion properties, thereby allowing the synthesis of microscope images of most biological samples. We first describe a method to integrate media dispersion into FDTD, and we validate the corresponding Angora dispersion module by applying Mie theory, as well as by experimentally imaging gold microspheres. Then, we demonstrate how Angora can facilitate the development of optical imaging techniques with a case study.

Paper Details

Date Published: 10 June 2016
PDF: 8 pages
J. Biomed. Opt. 21(6) 065004 doi: 10.1117/1.JBO.21.6.065004
Published in: Journal of Biomedical Optics Volume 21, Issue 6
Show Author Affiliations
Di Zhang, Northwestern Univ. (United States)
Ilker Capoglu, Northwestern Univ. (United States)
Yue Li, Northwestern Univ. (United States)
Lusik Cherkezyan, Northwestern Univ. (United States)
John Chandler, Northwestern Univ. (United States)
Graham Spicer, Northwestern Univ. (United States)
Hariharan Subramanian, Northwestern Univ. (United States)
Allen Taflove, Northwestern Univ. (United States)
Vadim Backman, Northwestern Univ. (United States)

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