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Journal of Biomedical Optics

ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems
Author(s): Veronica Sorgato; Michel Berger; Charlotte Emain; Christine Vever-Bizet; Jean-Marc Dinten; Geneviève Bourg-Heckly; Anne Planat-Chrétien
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Paper Abstract

We have developed an adaptive calibration algorithm and protocol (ACA-Pro) that corrects from the instrumental response of various spatially resolved diffuse reflectance spectroscopy (DRSsr) systems to enable the quantification of absorption and scattering properties based on a Monte Carlo-based look-up-table approach. The protocol involves the use of a calibration reference base built with measurements of a range of different diffusive intralipid phantoms. Moreover, an advanced strategy was established to take into account the experimental variations with an additional measurement of a common solid material, allowing the use of a single calibration reference base for all experiments. The ACA-Pro is validated in contact and noncontact probe-based DRSsr systems. Furthermore, the first results of a setup replacing the probe with a CCD detector are shown to confirm the robustness of the approach.

Paper Details

Date Published: 9 June 2016
PDF: 14 pages
J. Biomed. Opt. 21(6) 065003 doi: 10.1117/1.JBO.21.6.065003
Published in: Journal of Biomedical Optics Volume 21, Issue 6
Show Author Affiliations
Veronica Sorgato, CEA-LETI (France)
Michel Berger, CEA-LETI (France)
Charlotte Emain, CEA-LETI (France)
Christine Vever-Bizet, Univ. Pierre et Marie Curie (France)
CNRS (France)
Jean-Marc Dinten, MINATEC (France)
Geneviève Bourg-Heckly, Univ. Pierre et Marie Curie (France)
CNRS (France)
Anne Planat-Chrétien, CEA-LETI (France)

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