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Journal of Biomedical Optics • Open Access

Repeatability and reproducibility of optic nerve head perfusion measurements using optical coherence tomography angiography
Author(s): Chieh-Li Chen; Karine D. Bojikian; Chen Xin; Joanne C. Wen; Divakar Gupta; Qinqin Zhang; Raghu C. Mudumbai; Murray A. Johnstone; Philip P. Chen; Ruikang K. Wang

Paper Abstract

Optical coherence tomography angiography (OCTA) has increasingly become a clinically useful technique in ophthalmic imaging. We evaluate the repeatability and reproducibility of blood perfusion in the optic nerve head (ONH) measured using optical microangiography (OMAG)-based OCTA. Ten eyes from 10 healthy volunteers are recruited and scanned three times with a 68-kHz Cirrus HD-OCT 5000-based OMAG prototype system (Carl Zeiss Meditec Inc., Dublin, California) centered at the ONH involving two separate visits within six weeks. Vascular images are generated with OMAG processing by detecting the differences in OCT signals between consecutive B-scans acquired at the same retina location. ONH perfusion is quantified as flux, vessel area density, and normalized flux within the ONH for the prelaminar, lamina cribrosa, and the full ONH. Coefficient of variation (CV) and intraclass correlation coefficient (ICC) are used to evaluate intravisit and intervisit repeatability, and interobserver reproducibility. ONH perfusion measurements show high repeatability [CV≤3.7% (intravisit) and ≤5.2% (intervisit)] and interobserver reproducibility (ICC≤0.966) in all three layers by three metrics. OCTA provides a noninvasive method to visualize and quantify ONH perfusion in human eyes with excellent repeatability and reproducibility, which may add additional insight into ONH perfusion in clinical practice.

Paper Details

Date Published: 8 June 2016
PDF: 7 pages
J. Biomed. Opt. 21(6) 065002 doi: 10.1117/1.JBO.21.6.065002
Published in: Journal of Biomedical Optics Volume 21, Issue 6
Show Author Affiliations
Chieh-Li Chen, Univ. of Washington (United States)
Karine D. Bojikian, Univ. of Washington (United States)
Chen Xin, Univ. of Washington (United States)
Joanne C. Wen, Univ. of Washington (United States)
Divakar Gupta, Univ. of Washington (United States)
Qinqin Zhang, Univ. of Washington (United States)
Raghu C. Mudumbai, Univ. of Washington (United States)
Murray A. Johnstone, Univ. of Washington (United States)
Philip P. Chen, Univ. of Washington (United States)
Ruikang K. Wang, Univ. of Washington (United States)

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