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Journal of Biomedical Optics

Inflammation-related alterations of lipids after spinal cord injury revealed by Raman spectroscopy
Author(s): Sandra Tamosaityte; Roberta Galli; Ortrud Uckermann; Kerim H. Sitoci-Ficici; Maria Koch; Robert Later; Gabriele Schackert; Edmund Koch; Gerald Steiner; Matthias Kirsch
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Paper Abstract

Spinal cord injury (SCI) triggers several lipid alterations in nervous tissue. It is characterized by extensive demyelination and the inflammatory response leads to accumulation of activated microglia/macrophages, which often transform into foam cells by accumulation of lipid droplets after engulfment of the damaged myelin sheaths. Using an experimental rat model, Raman microspectroscopy was applied to retrieve the modifications of the lipid distribution following SCI. Coherent anti-Stokes Raman scattering (CARS) and endogenous two-photon fluorescence (TPEF) microscopies were used for the detection of lipid-laden inflammatory cells. The Raman mapping of CH2 deformation mode intensity at 1440  cm−1 retrieved the lipid-depleted injury core. Preserved white matter and inflammatory regions with myelin fragmentation and foam cells were localized by specifically addressing the distribution of esterified lipids, i.e., by mapping the intensity of the carbonyl Raman band at 1743  cm−1, and were in agreement with CARS/TPEF microscopy. Principal component analysis revealed that the inflammatory regions are notably rich in saturated fatty acids. Therefore, Raman spectroscopy enabled to specifically detect inflammation after SCI and myelin degradation products.

Paper Details

Date Published: 4 May 2016
PDF: 8 pages
J. Biomed. Opt. 21(6) 061008 doi: 10.1117/1.JBO.21.6.061008
Published in: Journal of Biomedical Optics Volume 21, Issue 6
Show Author Affiliations
Sandra Tamosaityte, TU Dresden (Germany)
Roberta Galli, Univ. Carl Gustav Carus Dresden (Germany)
Ortrud Uckermann, Univ. Carl Gustav Carus Dresden (Germany)
Kerim H. Sitoci-Ficici, Univ. Carl Gustav Carus Dresden (Germany)
Maria Koch, Univ. Carl Gustav Carus Dresden (Germany)
TU Dresden (Germany)
Robert Later, Univ. Carl Gustav Carus Dresden (Germany)
Gabriele Schackert, Univ. Carl Gustav Carus Dresden (Germany)
Edmund Koch, Univ. Carl Gustav Carus Dresden (Germany)
Gerald Steiner, Univ. Carl Gustav Carus Dresden (Germany)
Matthias Kirsch, Univ. Carl Gustav Carus Dresden (Germany)
TU Dresden (Germany)


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